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KMID : 1377020130100050273
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Tissue Engineering and Regenerative Medicine
2013 Volume.10 No. 5 p.273 ~ p.278
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In vitro effects of mussel-inspired polydopamine coating on Ti6Al4V alloy
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Lee Joon-Ho
Lim Young-Wook
Kwon Soon-Yong
Kim Yong-Sik
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Abstract
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Mussel adhesion phenomena in nature have inspired the integration of inorganic hydroxyapatite (HA) crystals within versatile materials. One example is the simple, aqueous, two-step functionalization approach, called polydopamine-assisted hydroxyapatite formation (pHAF), which consists of the chemical activation of material surfaces via polydopamine coating and the growth of hydroxyapatite in a simulated body fluid (SBF). For this study, we anticipated that such a polydopamine coating on the surface of titanium (Ti) alloy would improve the ability of cementless stems to osseointegrate. We compared the in vitro ability of cells to adhere to polydopamine-coated Ti alloy and machined Ti alloy. We performed energy-dispersive x-ray spectroscopy (EDS) and scanning electron microscopy (SEM) investigations to assess the structure and morphology of the surfaces. Biological and morphological responses to osteoblast cell lines (MC3T-E1) were then examined by measuring cell proliferation, cell differentiation (alkaline phosphatase (ALP) activity), and actin filament formation. Real-time polymerase chain reaction (PCR) was used to analyze gene expression for osteocalcin, osteonectin, and osteoprotegerin. Cell proliferation and ALP activity in the polydopamine-coated Ti alloy did not differ statistically compared to the other group. The polydopamine-coated Ti alloy exhibited better apatite formation ability than the untreated alloy, as evidenced by apatite formation after SBF immersion for 10 days. Molecular biological analysis did not differ statistically between the groups. The surface modification of the Ti alloy by coating with polydopamine did not change the biological properties of the Ti alloy. This may make some difficulties for osteogenesis signaling for the cells.
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KEYWORD
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polydopamine, Ti alloy, apatite
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